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光遺傳學(xué)&神經(jīng)學(xué)用激光器
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光遺傳學(xué)&神經(jīng)學(xué)用激光器

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光遺傳學(xué)&神經(jīng)學(xué)用激光器


光遺傳學(xué)是一個蓬勃發(fā)展的多學(xué)科生物工程技術(shù),結(jié)合了重組DNA技術(shù)與光學(xué)技術(shù),對細(xì)胞生物學(xué)的研究非常有用,具有獨(dú)特的高時空分辨率和細(xì)胞類型特異性的特點(diǎn),克服了傳統(tǒng)手段控制細(xì)胞或有機(jī)體活動的許多缺點(diǎn)。光遺傳學(xué)被廣泛應(yīng)用于活細(xì)胞內(nèi)目標(biāo)蛋白質(zhì)的跟蹤以及選擇性地控制腦中某類細(xì)胞的特定的神經(jīng)活動從而推動了神經(jīng)科學(xué)研究的深入。近來光遺傳學(xué)的應(yīng)用拓展到了信號傳導(dǎo)的研究,也開始有醫(yī)學(xué)臨床的應(yīng)用的報道。

神經(jīng)學(xué)是神經(jīng)系統(tǒng)的科學(xué)研究,是一門跨學(xué)科的科學(xué),與其他領(lǐng)域如化學(xué)、計(jì)算機(jī)科學(xué)、工程學(xué)、語言學(xué)、數(shù)學(xué)、醫(yī)學(xué)、遺傳學(xué)、哲學(xué)、物理學(xué)和心理學(xué)均有關(guān)聯(lián)。一些基于光學(xué)方法的研究技術(shù)已成為神經(jīng)科學(xué)領(lǐng)域的主要研究手段。神經(jīng)科學(xué)的研究范圍非常廣泛,包括神經(jīng)系統(tǒng)的功能、節(jié)后、發(fā)育、遺傳和病理學(xué)等方面,而其中對功能、結(jié)構(gòu)及功能與結(jié)構(gòu)關(guān)聯(lián)的研究更是核心內(nèi)容。在對神經(jīng)系統(tǒng)結(jié)構(gòu)的研究中,不斷進(jìn)步的光學(xué)成像技術(shù)一直發(fā)揮著重要作用。

長春新產(chǎn)業(yè)公司(CNI)是依托中科院長春光機(jī)所設(shè)立的企業(yè),主要從事激光器的研發(fā)與生產(chǎn)。公司先后通過了ISO9001認(rèn)證、歐洲CERoHS認(rèn)證、美國FDA認(rèn)證、日本JQA認(rèn)證。公司現(xiàn)可提供光基因遺傳用激光光源(自由空間或光纖耦合),光纖、植入式光纖插針、可旋轉(zhuǎn)光纖連接器、機(jī)械快門、聲光調(diào)制器、激光防護(hù)鏡等相關(guān)配件,以及整套測試系統(tǒng)。


特征

   CNI可提供自主研發(fā)生產(chǎn)的高可靠性激光光源;
    可提供光基因遺傳 與神經(jīng)學(xué)用測試系統(tǒng)的所有必須組件;
    易于安裝、拆卸及維護(hù);
    可提供客戶定制的解決方案。

光遺傳學(xué)常用激光器
紫外波段(nm):266   351   355   360   375
藍(lán)光波段(nm):405   435   442   445   447   457   460   465   473   488   491
綠光波段 (nm):515   520   532   543   556   561
黃光波段 (nm):589   593
紅光波段 (nm):633   637   640   642   650   671   690   720   750
紅外波段 (nm):785   800   808   825   830   905   915   940   980   1064 1342 1990   2200
多波長激光器 (nm):藍(lán)/ //
 
配件
fiber patch cables

光纖跳線

光纖插針

套管立體定位套管夾可旋轉(zhuǎn)光纖連接器

 AOM(Acousto-optic modulator)beam shutter
二合一激光耦合系統(tǒng)聲光調(diào)制器機(jī)械快門功率計(jì)防護(hù)鏡

使用CNI激光器取得的光遺傳學(xué)相關(guān)研究成果

(1) Optogenetic stimulation of a hippocampal engram activates fear memory recall
      (Nature, 484(7394): 381–385. doi:10.1038/nature11028)
(CNI-473nm)

(2) Ultrahigh accuracy imaging modality for super-localization microscopy
      (Nature Methods, VOL.10,2013,335)
(CNI-635nm and 488nm)

(3) Optogenetic silencing of locus coeruleus activity in mice impairs cognitive flexibility in an attentional set-shifting task
      (ORIGINALRESEARCH doi: 10.3389/fnbeh.2015.00286) (CNI-589nm)

(4) Optogenetic Evidence That Pallidal Projections, Not Nigral Projections, from the Nucleus Accumbens Core Are Necessary
       for Reinstating Cocaine Seeking (The Journal of Neuroscience, 2013?33(34):13654–13662)
(CNI-561nm)

(5) Control of a specific motor program by a small brain area in zebrafish
      (Front Neural Circuits, 2013; 7: 67) (CNI-405nm and 457nm)

(6) Fatal Neurological Respiratory Insufficiency Is Common Among Viral Encephalitides (Oxford Journals, 2013:208) (CNI-470nm)

(7) Temperature-dependent Activation of Neurons by Continuous Near-infrared Laser
      (Cell Biochemistry and Biophysics,vol.53, 33-42,2009)(CNI-1064nm)

(8) Distal connectivity causes summation and division across mouse visual cortex (Nature Neuroscience, 2014,Volume:17,Pages:

      30–32) (CNI-473nm)

(9) Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers
      (PNAS,2012,109(22),8483-8488) (CNI-980nm)


(10) Viral Vector-Based Techniques for Optogenetic Modulation In Vivo
       (Viral Vector Approaches in Neurobiology and Brain Diseases Neuromethods, Volume 82, 2014, pp 289-310) (CNI-447nm)

(11) Optogenetic Inhibition of Dorsal Medial Prefrontal Cortex Attenuates Stress-Induced Reinstatement of Palatable Food Seeking in
        Female Rats (The Journal of Neuroscience, 2013, 33(1):214–226) (CNI-593.5nm)

(12) Glutamatergic Signaling by Mesolimbic Dopamine Neurons in the Nucleus Accumbens
        (The Journal of Neuroscience, 2010, 30(20):7105-7110) (CNI-453nm)

(13) Firing of Hippocampal Neurogliaform Cells Induces Suppression of Synaptic Inhibition
        (The Journal of Neuroscience, 2014?34(4):1280–1292) (CNI-532nm)

(14) Intrinsic Electrophysiology of Mouse Corticospinal Neurons: a Class-Specific Triad of Spike-Related Properties
        (Oxford Journals, Volume 23,Issue 8,1965-1977) (CNI-473nm)

(15) Au–PLA nanocomposites for photothermally controlled drug delivery (J. Mater. Chem. B, 2014,2, 409-417)(CNI-808nm)

(16) Synthesis of magnetic, fluorescent and mesoporous core-shell-structured nanoparticles for imaging, targeting and photodynamic
        therapy (J. Mater. Chem., 2011,21, 11244-11252)(CNI-660nm)

(17) Asphaltene Adsorption onto an Iron Surface: Combined Near-Infrared (NIR), Raman, and AFM Study of the Kinetics,
        Thermodynamics and Layer Structure (Energy Fuels,2011,25(1), pp 189–196)(CNI-633nm)

(18) The electrophysiological properties of voltage-gated potassium channels on cultivated porcine retinal ganglion cells irradiated with
        continuous near-infrared laser (Chinese journal of ophthalmology 02/2012; 48(2):153-8).(CNI-845nm)

(19) High-resolution simultaneous voltage and Ca2+imaging (J Physiol February 1, 2011.589(3)489-494)(CNI-543nm)


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